Statistical quality indicators for electron-density maps

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Statistical quality indicators for electron-density maps

The commonly used validation metrics for the local agreement of a structure model with the observed electron density, namely the real-space R (RSR) and the real-space correlation coefficient (RSCC), are reviewed. It is argued that the primary goal of all validation techniques is to verify the accuracy of the model, since precision is an inherent property of the crystal and the data. It is demon...

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Probabilistic Methods for Interpreting Electron-Density Maps

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Bonding indicators from electron pair density functionals.

The bonding analysis of a chemical system is usually based on some descriptors. Distinct approaches are used to generate the bonding descriptors, whereby the usefulness of a particular approach is emphasized by the desire to yield a description consistent with the examined effects. Thus, whereas the bond path from the electron density gradient field yields the connectivity of the atomic fragmen...

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Experimental charge density from electron microscopic maps.

The charge density (CD) distribution of an atom is the difference per unit volume between the positive charge of its nucleus and the distribution of the negative charges carried by the electrons that are associated with it. The CDs of the atoms in macromolecules are responsible for their electrostatic potential (ESP) distributions, which can now be visualized using cryo-electron microscopy at h...

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Spherical-harmonic decomposition for molecular recognition in electron-density maps

Several methods for automatically constructing a protein model from an electron-density map require searching for many small protein-fragment templates in the density. We propose to use the spherical-harmonic decomposition of the template and the maps density to speed this matching. Unlike other template-matching approaches, this allows us to eliminate large portions of the map unlikely to matc...

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ژورنال

عنوان ژورنال: Acta Crystallographica Section D Biological Crystallography

سال: 2012

ISSN: 0907-4449

DOI: 10.1107/s0907444911035918